However, this occurred only in mice with large tumors and was not observed in the present study with tumors having a median tumor volume of 270 mm3. B, contour plot of CD8α‐PE‐Cy7 and Gr‐1‐PerCp‐Cy5.5 used to identify granulocytes, preGranulocyte gate, as Gr‐1+/CD8α‐ and memory T cells, Gr‐1 T cell gate, as Gr‐1+/CD8α+. In HIV+ patients (Figure 3A), pDCs were localized as in HIV− patients, in the marginal zone, in the periarteriolar T-cell area, and in the red pulp. In this experiment, the levels of DCs returned to near baseline by day 22. Vaccine 2002; 21: 322–329. © 2009 International Society for Advancement of Cytometry. Conflict-of-interest disclosure: The authors declare no competing financial interests. Evans TG, Hasan M, Galibert L, Caron D . Thus, both DC1s and DC2s were significantly increased in the spleen by i.v. The spleen and Peyer's patch cells were resuspended in 5 mL fluorescence activated cell‐sorting (FACS) buffer [0.1% sodium azide (Fisher Scientific, Pittsburgh, PA), 1.0% fetal bovine serum (Hyclone, Logan, UT) in Dulbecco's phosphate buffered saline, pH 7.4–7.6, sterile filtered]. This localization was similar in spleens from patients with idiopathic thrombopenic purpura (ITP) or hemolytic anemia (patients A, D, E, G, and K; Table 1, Figure 1A right panels), and from an atypical patient (patient B) with splenomegaly and angioma, as well as from organ donors (not shown). Blood 1996; 88: 2004–2012. We use cookies to help provide and enhance our service and tailor content and ads. The proviral loads from these 6 different patients varied from 0.96 to 723 HIV-1 DNA copies/million of spleen mononuclear cells, and they strongly correlated with pDC density (Figure 2B, ρ = 0.99, P = .003). BALB/c mice with cl-66 tumors growing in the mammary fat pad (median of 230 mm3 volume) were injected i.v. There was no significant difference in tumor volume. There have been reports of both one common or two separate precursor DC populations present in the mouse (7, 8). injection with 1 × 1011 Adv-Flt3L VPs. If you do not receive an email within 10 minutes, your email address may not be registered, Compensation was preformed before each data collection using BD Bioscience anti‐rat κ CompBeads according to manufacturer's recommendations. with Adv-Flt3L. (D) Endocytosis capacity of spleen pDCs and mDCs. I, Boolean logic used for hierarchal gating, including exclusion gates. V. Purification of spleen dendritic cells, new surface markers, and maintenance in vitro, Natural adjuvants: Endogenous activators of dendritic cells, Granulocyte‐dendritic cell unbalance in the non‐obese diabetic mice, Physiological concentrations of retinoic acid favor myeloid dendritic cell development over granulocyte development in cultures of bone marrow cells from mice, 1,25‐Dihydroxyvitamin D3 selectively modulates tolerogenic properties in myeloid but not plasmacytoid dendritic cells, In vitro induction of mucosa‐type dendritic cells by all‐trans retinoic acid, Estradiol acts directly on bone marrow myeloid progenitors to differentially regulate GM‐CSF or Flt3 ligand‐mediated dendritic cell differentiation, The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from different tissues including thymus, Characterization of murine lung dendritic cells: Similarities to Langerhans cells and thymic dendritic cells, Murine respiratory tract dendritic cells: Isolation, phenotyping and functional studies, Identification of plasmacytoid pre‐dendritic cells by one‐color flow cytometry for phenotype screening, Phenotypic comparison of multiple monocyte‐related populations in murine peripheral blood and bone marrow, Delineation among eight major hematopoietic subsets in murine bone marrow using a two‐color flow cytometric technique, Accurate and simple discrimination of mouse pulmonary dendritic cell and macrophage populations by flow cytometry: Methodology and new insights, Selecting fluorochrome conjugates for maximum sensitivity, Successful elimination of memory‐type CD8+ T cell subsets by the administration of anti‐Gr‐1 monoclonal antibody in vivo, Phenotype and function of intestinal dendritic cells, CD103‐ and CD103+ bronchial lymph node dendritic cells are specialized in presenting and cross‐presenting innocuous antigen to CD4+ and CD8+ T cells, Respiratory Syncytial Virus induced activation and migration of respiratory dendritic cells and subsequent antigen presentation in the lung draining lymph node, Novel subset of CD8[alpha]+ dendritic cells localized in the marginal zone is responsible for tolerance to cell‐associated antigens, A subfraction of B220(+) cells in murine bone marrow and spleen does not belong to the B cell lineage but has dendritic cell characteristics, Gender dimorphism of macrophage response to GMCSF and IL‐4 for differentiation into dendritic cells, Estrogen: A novel therapeutic adjunct for the treatment of trauma‐hemorrhage‐induced immunological alterations, Aging mice exhibit a functional defect in mucosal dendritic cell response against an intracellular pathogen, Aging impairs IFN regulatory factor 7 up‐regulation in plasmacytoid dendritic cells during TLR9 activation, Mouse strain differences in plasmacytoid dendritic cell frequency and function revealed by a novel monoclonal antibody, Spectral compensation for flow cytometry: Visualization artifacts, limitations, and caveats, A new “Logicle” display method avoids deceptive effects of logarithmic scaling for low signals and compensated data, A practical approach to multicolor flow cytometry for immunophenotyping, Seventeen‐colour flow cytometry: Unravelling the immune system. 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