Go to cart page 1X PBS with 1% BSA. 2.
The CofA will be sent to you by email with 1 to 3 business days. (Optional) After transfer, wash nitrocellulose membrane with 25 ml TBS for 5 minutes at room temperature. Lyse cells by adding 1X SDS sample buffer (100 μl per well of 6-well plate or 500 μl per plate of 10 cm diameter plate). Treat cells by adding fresh media containing regulator for desired time. Each bottle of dry-blend powder will make 1 L of ready to use buffer. 10X Tris Buffered Saline (TBS): (#9997) To prepare 1 liter of 10X TBS: 24.2 g Tris base, 80 g NaCl; adjust pH to 7.6 with HCl (use at 1X). For a solution of 1% BSA in PBS, add 1 g of BSA (Fraction V) to 100 ml of PBS. Products available from Cell Signaling Technology are linked by their respective catalog numbers.
1X SDS Sample Buffer: (#7722, #7723) 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue or phenol red. Heat a 20 μl sample to 95–100°C for 5 minutes; cool on ice.
During these difficult times, Boston BioProducts will continue to provide our customers and protect our employees during the COVID-19 outbreak. Aspirate media from cultures; wash cells with 1X PBS; aspirate. Continue shopping.
1X Phosphate Buffered Saline (PBS). Western Blotting Protocol (Primary Ab Incubation In BSA). 4.
#P1391 * 6 Bottle Minimum * Add to Cart. Blocker BSA is a 10% (w/v) solution of high-quality BSA that is useful for saturating excess protein-binding sites on membranes and microplates in immunoassays. For the best experience on our site, be sure to turn on Javascript in your browser. Keep on ice. 3. Use fom below to request a Certificate of Analysis. To make 1 L of 1X phosphate-buffered saline with Tween ® detergent (PBST), add 100 mL of 10X PBS and 1 mL of Tween ® detergent to 900 mL of water. Formulation. To prepare 0.1% (w/v) BSA fraction V in PBS, resuspend 10 mg of BSA fraction V (Sigma) in 10 ml of Ca 2+ /Mg 2+-free PBS to a final concentration of 0.1% (w/v).Filter through … If you want to be more exact (i.e. #P1391 * 6 Bottle Minimum *. Temperature Sensitive Items Must Be Shipped Overnight. Incubate membrane in 25 ml of blocking buffer for 1 hour at room temperature.
This PBST recipe contains 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4, and 1.8 mM KH 2 PO 4.. Incubate membrane with HRP-Streptavidin (at the appropriate dilution) in milk for one hour with gentle agitation at room temperature. NOTE:Prepare solutions with Milli-Q or equivalently purified water. Electrotransfer to nitrocellulose or PVDF membrane. Mathematically, one divided by 100 equals 1%. 1X PBS with 1% BSA. Temperature Sensitive Items Must Be Shipped Overnight.
5. Product was successfully added to your shopping cart. compensate for any volume change with the addition of BSA powder), you can add the 5 grams of powder to a smaller volume, and then top the volume off to 500 mL. As a critical manufacturer of chemicals, buffers, and reagents, we will take the necessary precautions to support both our OEM partners and research customers in their fight against COVID-19 and continuation of necessary research. Transfer Buffer:25 mM Tris base, 0.2 M glycine, 20% methanol (pH 8.5). 1000mL, Sterile. NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.